منابع مشابه
Stem Cell Organoid Engineering
Organoid systems leverage the self-organizing properties of stem cells to create diverse multicellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids...
متن کاملOrganoid technologies meet genome engineering.
Three-dimensional (3D) stem cell differentiation cultures recently emerged as a novel model system for investigating human embryonic development and disease progression in vitro, complementing existing animal and two-dimensional (2D) cell culture models. Organoids, the 3D self-organizing structures derived from pluripotent or somatic stem cells, can recapitulate many aspects of structural organ...
متن کاملVascularization in tissue engineering: angiogenesis versus inosculation.
BACKGROUND/PURPOSE The key challenge in tissue engineering is the establishment of an efficient vascularization for tissue constructs guaranteeing long-term survival and function. Vascularization may be achieved by the stimulation of angiogenesis or the inosculation of preformed microvascular networks within the implants to the host microvasculature. The present review provides an overview of t...
متن کاملA process engineering approach to increase organoid yield.
Temporal manipulation of the in vitro environment and growth factors can direct differentiation of human pluripotent stem cells into organoids - aggregates with multiple tissue-specific cell types and three-dimensional structure mimicking native organs. A mechanistic understanding of early organoid formation is essential for improving the robustness of these methods, which is necessary prior to...
متن کاملEfficient in vivo vascularization of tissue-engineering scaffolds.
The success of tissue engineering depends on the rapid and efficient formation of a functional blood vasculature. Adult blood vessels comprise endothelial cells and perivascular mural cells that assemble into patent tubules ensheathed by a basement membrane during angiogenesis. Using individual vessel components, we characterized intra-scaffold microvessel self-assembly efficiency in a physiolo...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2019
ISSN: 2296-4185
DOI: 10.3389/fbioe.2019.00039